The role of histamine in the retina: Studies on the HDC knockout mouse

U Greferath; KA Vessey; AI Jobling; SA Mills; BV Bui; Z He; N Nag; H Ohtsu; EL Fletcher

Journal article

The role of histamine in the retina: Studies on the HDC knockout mouse

U Greferath, KA Vessey, AI Jobling, SA Mills, BV Bui, Z He, N Nag, H Ohtsu, EL Fletcher

Plos One | Published : 2014

DOI: 10.1371/journal.pone.0116025

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Abstract

The role of histamine in the retina is not well understood, despite it regulating a number of functions within the brain, including sleep, feeding, energy balance, and anxiety. In this study we characterized the structure and function of the retina in mice that lacked expression of the rate limiting enzyme in the formation of histamine, histidine decarboxylase (Hdc-/- mouse). Using laser capture microdissection, Hdc mRNA expression was assessed in the inner and outer nuclear layers of adult C57Bl6J wildtype (WT) and Hdc-/--retinae. In adult WTand Hdc-/--mice, retinal fundi were imaged, retinal structure was assessed using immunocytochemistry and function was probed by electroretinography. Bl..

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University of Melbourne Researchers

Una Greferath Author

Kirstan Vessey Author

Andrew Jobling Author

Bang Bui Author

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Grants

Awarded by National Health and Medical Research Council (Australia)

Funding Acknowledgements

Funding provided by Grant #APP1021918 from the National Health and Medical Research Council (Australia) to ELF, UG and a grant from Retina Australia to ELF, UG. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.